温文玉
博士,研究员,博士生导师
地址:东安路131号,明道楼915室,上海200032
电话: 021-54237501 (办公室),021-54237502 (实验室)
工作经历
兼职教授复旦大学附属华山医院,神经外科,(2016.6-今)
研究员复旦大学,生物医学研究院,(2012.12-今)
副研究员复旦大学,生物医学研究院,(2009.1-2012.11)
博士后香港科技大学,生物化学系(2008.2-2008.12)
教育经历
博士 香港科技大学,生物化学系(2003.9-2008.1)
学士 复旦大学,化学系(1999.9-2003.7)
所获人才项目
国家优秀青年科学基金,国家自然科学基金,2014
新世纪优秀人才,教育部,2012
青年拔尖人才,上海市委组织部,2016
曙光学者,上海市教委,2014
青年科技启明星,上海市科委,2010
主要学术任职
中国生物物理学会生物磁共振专业委员会委员(2014.4-今)
中国生物物理学会女科学家分会理事(2015.11-今)
研究方向
综合结构生物学(核磁共振、X射线晶体衍射)、生物化学、细胞生物学及模式生物等多种手段,研究神经早期发育及神经信号转导过程中重要调控蛋白质的结构与功能,以及其与神经系统疾病(如脑肿瘤)的关联性。发表第一或通讯作者SCI论文21篇,包括Cell(封面文章),Mol Cell(2篇),Nat Commun(2篇),EMBO J(2篇)等。比较系统地研究了神经干细胞不对称分裂过程的分子机制,并修正了原有模型(Mol Cell, 2010;Mol Cell, 2011;EMBO J, 2011;JMB, 2013;Nat Commun, 2015;Structure, 2016;EMBO Rep, 2017;JBC, 2018a;JBC, 2018b;Nat Commun, 2018);提出了视觉支架蛋白INAD主动调节自身氧化还原电势进而介导信号传导过程的新机制(Cell, 2011, 封面文章)。主持国家及省部级项目10余项,含国家重大科学研究计划课题1项、国家自然科学基金项目5项。
代表性论文(*为通讯作者):
Shan, Z., Tu, Y., Yang, Y., Liu, Z., Zeng, M., Xu, H., Long, J., Zhang, M., Cai, Y.*, and Wen, W.*“Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division”Nat. Commun. (2018) 9:737.
Yao, W., Shan, Z., Gu, A., Fu, M., Shi, Z., and Wen, W.* “WW domain-mediated regulation and activation of E3 ubiquitin ligase Suppressor of Deltex” J. Biol. Chem. (2018) 293, 16697-16708.
Chen, X., Liu, Z., Shan, Z., Yao, W., Gu, A., and Wen, W.*“Structural determinants controlling 14-3-3 recruitment to the endocytic adaptor Numb and dissociation of the Numb/α-adaptin complex” J. Biol. Chem. (2018) 293, 4149-4158.
Zhu, K., Shan, Z., Chen, X., Cai, Y., Cui, L., Yao, W., Wang, Z., Shi, P., Tian, C., Lou, J., Xie, Y., and Wen, W.* “Allosteric auto-inhibition and activation of the Nedd4 family E3 ligase Itch” EMBO Rep. (2017) 18, 1618-1630.
Zhu, K., Shan, Z., Zhang, L., and Wen, W.* “Phospho-Pon binding mediated fine-tuning of Plk1 activity” Structure (2016) 24, 1110-1119.
Jia, M., Shan, Z., Yang, Y., Liu, C., Li, J., Luo, Z.-G., Zhang, M., Cai, Y.*, Wen, W.*, and Wang, W.* “The structural basis of Miranda-mediated Staufen localization duringDrosophila neuroblast asymmetric division” Nat. Commun. (2015) 6:8381.
Pan, Z., Shang, Y., Jia, M., Zhang, L., Xia, C., Zhang, M., Wang, W.*, and Wen, W.* “Structural and biochemical characterization of the interaction between LGN and Frmpd1”J. Mol. Biol. (2013) 425, 1039-1049.
Zhu, J., Shang, Y., Xia, C., Wang, W., Wen, W.*, and Zhang, M.* “Guanylate Kinase Domains of the MAGUK Family Scaffold Proteins as Specific Phospho-protein-binding Modules” EMBO J. (2011) 30, 4986-4997.
Liu, W.#, Wen, W.#, Wei, Z., Yu, J., Ye, F., Liu, C.-H., Hardie, R.C., and Zhang, M. “The INAD scaffold is a dynamic, redox-regulated modulator of signaling in the Drosophila eye” Cell (2011) 145, 1088-1101. (#: co-first authors) (Cover article)
Highlights: F1000,http://f1000.com/prime/12082956
Zhu, J.#, Wen, W.#, Zheng, Z., Shang, Y., Wei, Z., Xiao, Z., Pan, Z., Du, Q., Wang, W., and Zhang, M. “LGN/mInsc and LGN/NuMA complex structures suggest distinct functions in asymmetric cell division for the Par3/mInsc/LGN and Gαi/LGN/NuMA pathways” Mol. Cell(2011) 43, 418-431. (#: co-first authors)
Wen, W., Yu, J., Pan, L., Wei, Z., Weng, J., Wang, W., Ong, Y.S., Hoai, T.T.T., Hong, W., and Zhang, M. “Lipid-induced conformational switch controls fusion activity of longin domain SNARE Ykt6”Mol. Cell(2010) 37, 383-395.
Highlights: F1000, http://f1000.com/prime/2939958